Evapotranspiration and crop coefficient patterns of an apple orchard in a sub-humid environment

Abstract Increasing water use efficiency is one of the main challenges of sustainable fruit tree production. From 2013 to 2015 we measured actual evapotranspiration (ETa) using eddy covariance in a well-irrigated apple orchard located in in South Tyrol (Italy), a sub-humid environment. We assessed the experimental crop coefficient ( K c e x p ) and analyzed the dependency of Kc on specific environmental variables at a daily time scale. K c e x p values changed throughout the season following a bell-shaped trend and were generally lower than the FAO tabular values corrected for local climatic conditions. In the mid-season phase, when LAI and tabular Kc are supposed to be constant, the average experimental Kc ( K c ¯ e x p ) was 1.01, 86% of the Kc value reported by FAO (1.18). Mid-season Kc residuals ( K c e x p - K c ¯ e x p ) were positively correlated with daily vapor pressure deficit (VPD) (ρ = 0.45), suggesting that the daily Kc variability observed is due, at least in part, to changes in the evaporative demands of the atmosphere. We explain these results by considering the relatively humid environment, the high water availability and the fact that leaves on apple trees are more tightly coupled to the atmosphere with respect to a smoother grass surface

Contribution of cauliflower residues to N nutrition of subsequent lettuce crops grown in rotation in an Italian Alpine environment

Up to 75% of nitrogen (N) taken up during cauliflowers production is allocated to leaves, which are left as crop residues after harvest. The inclusion of cauliflower, cultivated alone or intercropped with legumes, in rotation schemes, is a promising tool to optimize N availability to subsequent crops. This original study assessed, for the first time in South Tyrol, Italy, the effect of removal or soil incorporation of cauliflower and clover residues on the growth and N uptake of subsequent lettuce. In 2015, cauliflower was sole-cropped or intercropped with clover, under different N regimes (N0, N1, N2, N3 = 0, 75, 150, 300 kg N ha−1). Cauliflower and clover residues were either removed or incorporated in the soil in 2016. The effects of the residual fertility left by the N fertilizer, the two cropping systems, and the crop residues were assessed on the yield and N uptake of two subsequent lettuce crops. Isotopic 15Nlabeled cauliflower residues were additionally used to quantify the N contribution of cauliflower residues to lettuce growth. During the first lettuce crop, residues incorporation was the only factor increasing lettuce yields (+41%) and N uptake (+58%). The residual effect of N1 and N2 rates increased the lettuce N uptake when clover residues were incorporated. During the second lettuce crop, residues incorporation increased lettuce yields (+26%) and N uptake (+44%). On average, 64% and 35% of the lettuce N amounts, in the first and second cycles, respectively, derived from cauliflower residues, and accounted for 38% of the total N contained in cauliflower residues (214 kg N ha−1). Results from this experiment, uncommon for the examined species, demonstrate that incorporation of cauliflower and clover residues provides an excellent source of N for lettuce. Incorporating residues of the preceding cauliflower crop, alone or intercropped with clover, before establishing the lettuce crop, substantially reduce the N fertilization needs of subsequent lettuce crops

Intra- and Intertree Variability of the 87Sr/86Sr Ratio in Apple Orchards and Its Correlation with the Soil 87Sr/86Sr Ratio.

The 87Sr/86Sr ratio of horticultural products mostly derives from that of the bioavailable Sr fraction of the soil where they grow and, therefore, varies according to the local geolithological features. This study investigated the intra- and intertree variability of the 87Sr/86Sr ratio in two apple orchards in South Tyrol and its relation with the soil 87Sr/86Sr ratio. In both orchards, a moderate homogeneity of the 87Sr/86Sr ratio was observed among subsamples of the same tree part (shoot axes, leaves, apple peels, and pulps). Moreover, the 87Sr/86Sr ratio homogeneity among tree parts was high intratree and low intertree. The variability of the 87Sr/86Sr ratio within the tree and within the orchard is explained in light of the 87Sr/86Sr ratios of the soil. This 87Sr/86Sr variability within orchards does not preclude its use as a geographical tracer; however, this aspect should be evaluated to correctly design a sampling campaign or to generalize the results

Chemical and biological indicators of water quality in three agricultural watersheds of the Po valley, Italy

open9noAgriculture has both direct and indirect effects on quality of surface water and is one of the key activities causing water quality degradation. Its environmental impact can be evaluated by the determination of indicators of the quality of water bodies that collect drainage and runoff waters from agricultural watersheds. For this research, the water quality draining from three watersheds, totally or partially cultivated, all within the Po river valley (Italy), was determined, using chemical indicators (N-NO3 and N-NH4 concentration, N balance), trophic status (chlorophyll-a concentration) and benthic population indexes. Together, they should provide an overview of the water status, which is supposed to be strictly related to the land use and the management. Results show that the chemical parameters are well related to land use and farming management: intensive agricultural activity leads to high N-NO3 concentration in water and N surplus and vice versa. The chlorophyll-a concentration follows the same trend, being linked to nitrogen loads and land use. Not always there is accordance between chemical and biological indicators: no direct correspondence is evident between the N-NO3 concentration in waters and benthic community. Its presence and abundance seems to be mostly correlated with the geomorphology, hydrology, riparian strips, etc. of the habitat than to the land use. Only the integration of chemical and biological parameters allows a correct understanding of the state of health of water body and benthic communities.openPIERI L.; VENTURA F.; VENTURA M.; TAGLIAVINI M.; PONTI M.; PISTOCCHI R.; ALBERTAZZI S.; VIGNUDELLI M.; ROSSI PISA P.PIERI L.; VENTURA F.; VENTURA M.; TAGLIAVINI M.; PONTI M.; PISTOCCHI R.; ALBERTAZZI S.; VIGNUDELLI M.; ROSSI PISA P

How do geomorphic characteristics affect the source of tree water uptake in restored river floodplains?

Alpine rivers and their floodplains have been highly modified by human activities during the last decades. River restoration projects aim to counteract these negative impacts and to restore ecosystem services provided by riparian habitats. We studied two recently restored river sites in the Ahr/Aurino and Mareit/Mareta Rivers (Italian Alps) to investigate how geomorphic conditions, soil moisture, and groundwater level affect the source of water used by grey alder (Alnus incana (L.) Moench). We compared the isotopic composition (δ2H) of tree sap at different locations (low terraces formed during bed incision and recent floodplains formed after restoration) with that of potential water sources, that is, groundwater, soil water, and rainfall. The monthly variation in the isotopic composition of rainfall was reflected in both shallow and deeper soil water, as well as in the isotopic composition of sap. The redistribution of precipitation and groundwater in the soil differed between the post-restoration floodplain sites and the post-incision terraces, leading to a different relation between the sap water, soil water, and groundwater isotopic composition. The results show that transpiration of A. incana trees growing on recent floodplains is mostly supported by stream-fed soil water, whereas trees growing on terraces mainly use precipitation-fed soil water. These marked, morphology-related differences in the source of transpiration water of grey alder highlight how channel degradation still affects the ecohydrological processes in Alpine fluvial corridors. Nonetheless, large restoration interventions—in terms of channel widening—can enable the self-formation of new floodplain areas characterized by stream water-fed riparian ecosystems

Recent achievements and new research opportunities for optimizing macronutrient availability, acquisition, and distribution for perennial fruit crops

CITATION: Kalcsits, Lee et al. 2020. Recent achievements and new research opportunities for optimizing macronutrient availability, acquisition, and distribution for perennial fruit crops. Agronomy, 10(11): 1738, doi.org/10.3390/agronomy10111738.The original publication is available at: https://www.mdpi.comTree responses to fertilizer management are complex and are influenced by the interactions between the environment, other organisms, and the combined genetics of composite trees. Increased consumer awareness of the environmental impact of agriculture has stimulated research toward increasing nutrient-use efficiency, improving environmental sustainability, and maximizing quality. Here, we highlight recent advancements and identify knowledge gaps in nutrient dynamics across the soil–rhizosphere–tree continuum for fruit crops. Beneficial soil management practices can enhance nutrient uptake and there has been significant progress in the understanding of how roots, microorganisms, and soil interact to enhance nutrient acquisition in the rhizosphere. Characterizing root architecture, in situ, still remains one of the greatest research challenges in perennial fruit research. However, the last decade has advanced the characterization of root nutrient uptake and transport in plants but studies in tree fruit crops have been limited. Calcium, and its balance relative to other macronutrients, has been a primary focus for mineral nutrient research because of its important contributions to the development of physiological disorders. However, annual elemental redistribution makes these interactions complex. The development of new approaches for measuring nutrient movement in soil and plant systems will be critical for achieving sustainable production of high-quality fruit in the future.Publisher's versio

Treatment with human, recombinant FSH improves sperm DNA fragmentation in idiopathic infertile men depending on the FSH receptor polymorphism p.N680S: A pharmacogenetic study

Study question: Does the spermDNAfragmentation index (DFI) improve depending on the FSH receptor (FSHR) genotype as assessed by the nonsynonymous polymorphisms rs6166 (p.N680S) after 3 months of recombinant FSH treatment in men with idiopathic infertility? summary answer: FSH treatment significantly improves sperm DFI only in idiopathic infertile men with the p.N680S homozygous N FSHR. what is known already: FSH, fundamental for spermatogenesis, is empirically used to treat male idiopathic infertility and several studies suggest that DFI could be a candidate predictor of response to FSH treatment, in terms of probability to conceive. Furthermore, it is known that the FSHR single nucleotide polymorphism (SNP) rs6166 (p.N680S) influences ovarian response in women and testicular volume in men. study design, size and duration: Amulticenter, longitudinal, prospective, open-label, two-arm clinical trial was performed. Subjects enrolled were idiopathic infertile men who received 150 IU recombinant human FSH s.c. every other day for 12 weeks and were followed-up for a further 12 weeks after FSH withdrawal. Patients were evaluated at baseline, at the end of treatment and at the end of follow-up. participants/materials, setting, methods: Eighty-nine men with idiopathic infertility carrier of the FSHR p.N680S homozygousNor S genotype, FSH 64 8 IU/l and DFI >15%,were enrolled. A total of 66 patients had DFI analysis completed on at least two visits. DFI was evaluated in one laboratory by TUNEL/PI (propidium iodide) assay coupled to flow cytometry, resolving two different fractions of sperm, namely the 'brighter' and 'dimmer' sperm DFI fractions. main results and the roleof chance: Thirty-eightmen(57.6%)were carriers of the p.N680S homozygousNand 28 (42.4%) of the homozygous S FSHR. Sperm concentration/number was highly heterogeneous and both groups included men ranging from severe oligozoospermia to normozoospermia. Total DFI was significantly lower at the end of the study in homozygous carriers of the p.N680SNversus p.N680S S allele (P = 0.008). Total DFI decreased significantly from baseline to the end of the study (P = 0.021) only in carriers of the p.N680S homozygous N polymorphism, and this decrease involved the sperm population containing vital sperm (i.e. brighter sperm) (P = 0.008). The dimmer sperm DFI fraction, including only nonvital sperm, was significantly larger in p.N680S S homozygous patients than in homozygous N men (P = 0.018). Total DFIwas inversely related to total sperm number (P = 0.020) and progressive sperm motility (P = 0.014).Whenpatients were further stratified according to sperm concentration (normoozospermic versus oligozoospermic) or -211G>T polymorphism in the FSHB gene (rs10835638) (homozygous Gversus others), the significant improvement of sperm DFI in FSHR p.N680S homozygousNmen was independent of sperm concentration and associated with the homozygous FSHB -211G>T homozygous G genotype. limitations, reasons for caution: The statistical power of the study is 86.9% with alpha error 0.05. This is the first pharmacogenetic study suggesting that FSH treatment induces a significant improvement of total DFI in men carriers of the p.N680S homozygousNFSHR; however, the results need to be confirmed in larger studies using a personalized FSH dosage and treatment duration. wider implications of the findings: The evaluation of sperm DFI as a surrogate marker of sperm quality, and of the FSHR SNP rs6166 (p.N680S), might be useful to predict the response to FSH treatment in men with idiopathic infertility. study funding/competing interest(s): The study was supported by an unrestricted grant to M.S. and H.M.B. from Merck Serono that provided the drug used in the study. MS received additional grants from Merck Serono and IBSA as well as honoraria from Merck Serono. The remaining authors declare that no conflicts of interest are present. trial registration number: EudraCT number 2010-020240-35